Annals of Epidemiology
Volume 18, Issue 7 , Pages 572-578, July 2008

The Polymorphism of XRCC3 Codon 241 and AFB1-Related Hepatocellular Carcinoma in Guangxi Population, China

  • Xi Dai Long, MD

      Affiliations

    • Department of Pathology, Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region
    • Corresponding Author InformationAddress correspondence to: Xi Dai Long, MD, Chengxiang Road N0. 98, Baise City, Guangxi Zhuang Autonomous Region, China, 533000. Tel.: +86 776 2849481; fax: +86 776 2849481.
    • ,
  • Yun Ma, MD

      Affiliations

    • Department of Pathology, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region
    • ,
  • De Ying Qu, JS

      Affiliations

    • Department of Pathology, Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region
    • ,
  • Yun Guang Liu, JS

      Affiliations

    • Department of Medicine, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region
    • ,
  • Zhao Quan Huang, JS

      Affiliations

    • Department of Pathology, Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region
    • ,
  • Yong Zhi Huang, JS

      Affiliations

    • Department of Pathology, Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region
    • ,
  • Zhong Hui Lin, MD

      Affiliations

    • Department of Medicine, Zhong Hospital of Xiamen, Xiamen, Fujian Province
    • ,
  • Ni Bo Wei

      Affiliations

    • Department of Clinical Medicine (2004), Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region
    • ,
  • Shu Chun Zhou

      Affiliations

    • Department of Clinical Medicine, the Second Hospital of Anhua, Hu'nan Province, China

Received 31 October 2007; accepted 1 March 2008. published online 27 May 2008.

Purpose

The relationship between aflatoxin B1 (AFB1) exposure and hepatocellular carcinoma (HCC) has been previously demonstrated and supported with strong epidemiological evidence. However, the role of genetic polymorphism of X-ray cross-complementing group 3 (XRCC3) codon 241 (namely: Thr241Met), which may be involved in the repair of DNA double-strand breaks caused by carcinogens such as AFB1, been less well elaborated.

Methods

We conducted a case–control study including 491 cases and 862 controls to evaluate the associations between this polymorphism and HCC risk for Guangxi population by means of polymerase chain reaction-restriction fragment length polymorphism analysis.

Results

We found that individuals with the XRCC3 genotypes with codon 241 Met (namely XRCC3-TM or XRCC3-MM) had an increased risk of HCC than those with the homozygote of XRCC3 codon 241 Thr alleles (namely XRCC3-TT, adjusted odds ratios 2.22 and 7.19; 95% confidence intervals 1.72–2.88 and 4.52–11.42, respectively). The risk of HCC, moreover, did appear to differ more significantly among individuals featuring high-level AFB1-DNA adducts, whose adjusted odds ratios (95% confidence intervals) were 11.59 (5.73–23.47) and 37.54 (16.32–86.32), respectively.

Conclusions

These findings support the hypothesis that the XRCC3 Thr241Met polymorphism may be associated with the risk of AFB1-related HCC among the Guangxi population.

Key Words: HCC, XRCC3, Polymorphism, AFB1

Selected Abbreviations and Acronyms: HCC, hepatocellular carcinoma, AFB1, aflatoxin B1, SSB, single-strand breaks, DSB, double-strand breaks, HRR, homologous recombination repair, XRCC3, X-ray repair cross-complementing group 3, XRCC3-MM, the homozygotes of XRCC3 codon 241 Met alleles, XRCC3-TM, the heterozygotes of XRCC3 codon 241 Met and Thy allele, XRCC3-TM/MM, the XRCC3 genotypes with codon 241 Met alleles, XRCC3-TT, the homozygote of XRCC3 codon 241Thr alleles, HBV, hepatic B virus, HCV, hepatic C virus, HBsAg, hepatitis B surface antigen, PCR, polymerase chain reaction, RFLP, restriction fragment length polymorphism, 95% CI, confidence interval, OR, odds ratio

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 Drs. Long and Ma contributed equally to this work.

 This study is supported by the Science Foundation of Youjiang Medical College for Nationalities (NO. 2005 and 2008) and the National Nature & Science Foundation of China (NO. 39860032).

PII: S1047-2797(08)00058-6

doi:10.1016/j.annepidem.2008.03.003

Annals of Epidemiology
Volume 18, Issue 7 , Pages 572-578, July 2008

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